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SCI1 是细胞增殖的花调节因子，其伴侣 NtCDKG2 和 NtRH35 与剪接机制相互作用

日期：2025-04-19 00:02:04 点击： 属于：知识角

SCI1 是细胞增殖的花调节因子，其伴侣 NtCDKG2 和 NtRH35 与剪接机制相互作用
Journal of Experimental Botany ( IF 5.6 ) Pub Date : 2024-08-08 , DOI: 10.1093/jxb/erae337
Vitor F Pinoti 1, 2 , Pedro B Ferreira 1, 2 , Edward J Strini 1, 2 , Greice Lubini 1, 2 , Vanessa Thomé 1, 2 , Joelma O Cruz 1, 2 , Rodrigo Aziani 1 , Andréa C Quiapim 1 , Andressa P A Pinto 3 , Ana Paula U Araujo 3 , Henrique C De Paoli 1, 2 , Maria Cristina S Pranchevicius 4 , Maria Helena S Goldman 1, 2

Affiliation

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, Brazil.
PPG-Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, Brazil.
São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil.
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil.

成功的植物繁殖取决于受细胞增殖和其他过程控制的花器官的充分发育。柱头/型细胞周期抑制剂 1 （SCI1）基因调节细胞增殖并影响女性生殖器官的最终大小。为了揭示烟草 SCI1 在细胞增殖控制中的分子机制，我们通过半体内沉降实验寻找其相互作用伙伴，发现了一种细胞周期蛋白依赖性激酶 NtCDKG;2。双分子荧光互补和共定位实验表明，SCI1 与 NtCDKG 相互作用;2 及其同源 NtCyclin L 在核仁和剪接斑点中。使用 SCI1 作为诱饵筛选酵母双杂交 cDNA 文库揭示了一种新的 DEAD-box RNA 解旋酶（NtRH35）。NtCDKG 之间的相互作用;还显示了 2-NtCyclin L 复合物和 NtRH35。亚细胞定位实验显示，SCI1 、 NtRH35 和 NtCDKG;2–NtCyclin L 复合物在剪接斑点内彼此结合。NtCDKG 的酵母双杂交筛选;2 和 NtRH35 鉴定了保守的剪接体组分 U2aʹ、NF-κB 激活蛋白（NKAP）和 CACTIN。这项工作介绍了 SCI1 及其交互因子 NtCDKG;2–NtCyclin L 复合物和 NtRH35，作为新的剪接体相关蛋白。我们的研究结果揭示了一个相互作用网络，并表明 SCI1 可能通过剪接过程调节细胞增殖，为控制植物发育的复杂分子途径提供了新的见解。

SCI1, a flower regulator of cell proliferation, and its partners NtCDKG2 and NtRH35 interact with the splicing machinery

Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2–NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2–NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2aʹ, NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2–NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development.

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SCI1 是细胞增殖的花调节因子，其伴侣 NtCDKG2 和 NtRH35 与剪接机制相互作用

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