PbrATG6 调节活性氧代谢并与 PbrTLP15 相互作用，协同增强梨对 Botryosphaeria dothidea 的抗性
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2024-10-17 , DOI: 10.1016/j.ijbiomac.2024.136663
Yun Wang 1 , Yuting Liu 1 , Yue Zhang 1 , Xiaolei Sun 1 , Fei Wang 1 , Zhihua Xie 1 , Kaijie Qi 1 , Xun Sun 1 , Shaoling Zhang 1
自噬对于植物防御病原体至关重要，ATG6 是这一过程中的关键基因。目前，关于 ATG6 介导的梨病原菌耐药的潜在功能和分子机制的报道很少。本研究探讨了 ATG6 的梨同源物 （PbrATG6） 在对 Botryosphaeria dothidea 的抗性中的作用。PbrATG6 在梨组织中差异表达，感染后其表达增加。PbrATG6 的过表达增强了拟南芥和梨愈伤组织的抗性，而沉默则增加了敏感性。PbrTLP15 是一种属于 PR5 家族的发病机制相关蛋白，通过酵母双杂交筛选发现它与 PbrATG6 相互作用。酵母双杂交、荧光素酶互补成像、双分子荧光互补测定和沉降测定显示 PbrATG6 与 PbrTLP15 相互作用。PbrATG6 和 PbrTLP15 的瞬时沉默转基因实验显示，PbrATG6 可以与 PbrTLP15 协同调控梨 B. dothidea 抗性。此外，pTRV-PbrTLP15 和透射电子显微镜 （TEM） 测定中自噬关键基因的转录分析也表明 PbrTLP15 确实影响自噬。因此，PbrATG6 和 PbrTLP15 可以协同增强梨 B. dothidea 病的抵抗力。它为梨病抗性中自噬的研究提供了新的策略，丰富了梨病抗性机制的研究。
PbrATG6 modulates reactive oxygen species metabolism and interacts with PbrTLP15 synergistic enhancement of pear resistance to Botryosphaeria dothidea
Autophagy is vital for plant defense against pathogens, with ATG6 being a key gene in this process. At present, little has been reported on the potential function and molecular mechanisms of ATG6 mediated pathogen resistance in pear. This study investigates the function of the pear homolog of ATG6 (PbrATG6) in resistance to Botryosphaeria dothidea. PbrATG6 is expressed differentially in pear tissues and its expression increases upon infection. Overexpression of PbrATG6 enhances resistance in Arabidopsis and pear calli, while silencing it increases susceptibility. PbrTLP15, a pathogenesis-related protein belonging to the PR5 family, was found that interacts with PbrATG6 by a yeast two-hybrid screening. Yeast two-hybrid, luciferase complementation imaging, bimolecular fluorescence complementation assays and pull-down assays showed that PbrATG6 interacts with PbrTLP15. The transient silencing transgenic assays of PbrATG6 and PbrTLP15 revealed that PbrATG6 could cooperate with PbrTLP15 to regulate pear B. dothidea resistance. In addition, transcriptional analyses of autophagy key genes in pTRV-PbrTLP15 and transmission electron microscopy (TEM) assays also implied that PbrTLP15 does affect autophagy. Hence, PbrATG6 and PbrTLP15 may synergistically enhance pear B. dothidea disease resistance. It provides a new strategy for the study of autophagy in pear disease resistance and enriches the research on pear disease resistance mechanism.
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