HB52-PUT2 模块介导的多胺芽到根的运动调节番茄的盐胁迫耐受性
Plant, Cell & Environment ( IF 6.0 ) Pub Date : 2025-04-01 , DOI: 10.1111/pce.15479
Xian Yang 1 , Hongyi Qin 1 , Yu Zhou 1 , Ziqi Mai 1 , Xirong Chai 1 , Juxian Guo 2 , Yunyan Kang 1 , Min Zhong 1
Affiliation
College of Horticulture, South China Agricultural University, Guangzhou, China.
Vegetable Research Institute, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
土壤盐分严重限制了作物的质量和产量。植物已经开发出各种策略来减轻盐胁迫的负面影响，包括多胺摄取转运蛋白 （PUT） 对多胺的重新分配。然而，PUT 在盐胁迫期间改变多胺易位过程的机制尚未完全阐明。在这里，我们表明参与多胺芽到根运输的 PUT2 的破坏导致番茄中的盐敏感表型。此外，筛选与 PUT2 相互作用的 HD-Zip 转录因子 HB52 的酵母单杂交体，HB52 功能的丧失也导致对盐胁迫的敏感性增加，而 HB52 过表达系表现出更好的耐盐性。此外，分子分析表明，HB52 直接激活 PUT2 的表达，并通过促进多胺芽到根的迁移来促进 Na+ 外排。本研究揭示了与同源框蛋白调节因子相关的协同转录调控网络，该调节因子在盐胁迫下促进多胺的长距离运输。
HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
Soil salinity severely restricts crop quality and yields. Plants have developed various strategies to alleviate salinity stress's negative effects, including polyamine redistribution by polyamine uptake transporters (PUTs). However, the mechanisms by which PUTs alter polyamine translocation processes during salt stress have not been fully elucidated. Here, we show that disruption of PUT2, which is involved in polyamine shoot‐to‐root transport, results in salt sensitivity phenotypes in tomato. Moreover, yeast one‐hybrid screened for an HD‐Zip transcription factor HB52 that interacts with PUT2, and loss of function of HB52 also led to increased sensitivity to salt stress, whereas HB52‐overexpression lines exhibited improved salt tolerance. Furthermore, molecular analyses demonstrated that HB52 directly activated the expression of PUT2 and facilitated Na+ efflux by promoting polyamine shoot‐to‐root mobility. This study uncovers a synergistic transcriptional regulatory network associated with a homeobox protein regulator that promotes polyamine long‐distance transport under salt stress.
更新日期：2025-04-01
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