DkGASA4 在赤霉素调控的柿子果实采后软化中发挥作用
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2025-01-13 , DOI: 10.1016/j.plaphy.2025.109509
Ceng-Xi Xiong 1 , Yi-Hang Zhang 2 , Yi-Min Wang 2 , Shen-Yue Xie 2 , Hao-Tian Wu 2 , Hao-Miao Ding 2 , Wei Chen 2 , Li-Yu Shi 1 , Zhen-Feng Yang 3 , Wei Wu 3
Affiliation
Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, China.
Hwamei College of Life and Health Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, China.
Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, China; Hwamei College of Life and Health Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, China.
赤霉素 （GA） 是参与水果成熟调节的重要植物激素之一。响应 GA 并编码富含半胱氨酸的肽的 GASA 基因在植物中普遍存在。虽然 GASA 基因家族已在多种植物中被鉴定出来，但它在柿子果实成熟中的作用仍不清楚。本研究对柿子的 GASA 基因进行了鉴定和分析。我们发现赤霉素处理抑制 DkGASA4 的表达。此外，通过双荧光素酶和酵母双杂交测定，我们发现 DkGASA4 与 DkNAC9 相互作用以协同激活细胞壁降解基因 DkEGase1 的转录。本研究强调了 DkGASA4 在柿子果实成熟和软化中的重要调控功能。
DkGASA4 plays a role in the postharvest softening of persimmon fruit regulated by gibberellin
Gibberellin (GA) is one of the crucial plant hormones involved in fruit ripening regulation. GASA genes, which respond to GA and encode cysteine-rich peptides, are prevalent in plants. While the GASA gene family has been identified in various plants, its role in persimmon fruit ripening remains unclear. In this study, the GASA genes of persimmon were identified and analyzed. We found that gibberellin treatment suppresses the expression of the DkGASA4. Additionally, through dual-luciferase and yeast two-hybrid assays, we discovered that DkGASA4 interacts with DkNAC9 to synergistically activate the transcription of the cell wall degradation gene DkEGase1. This study highlights the significant regulatory function of DkGASA4 in persimmon fruit ripening and softening.
更新日期：2025-01-13
知识角