酵母双杂交文库筛选能够发现新的蛋白质-蛋白质相互作用。鉴定宿主转位疫霉菌效应蛋白靶向的胞质宿主蛋白依赖于用于制备酵母双杂交 cDNA 文库的 mRNA 数量、质量和组成。在这里，我们描述了制备针对酵母中疫霉菌效应子靶标筛选优化的 Pinus radiata cDNA 文库所需的步骤。这种方法也可以应用于其他寄主植物和病原体物种。
Pinus radiata cDNA Library for the Screening of Phytophthora Effector Protein Targets in Yeast.
Yeast two-hybrid library screening enables the discovery of novel protein-protein interactions. Identifying cytosolic host proteins targeted by host-translocated Phytophthora effector proteins relies on the mRNA amount, quality, and composition used to prepare the yeast two-hybrid cDNA library. Here we describe the steps required for the preparation of a Pinus radiata cDNA library optimized for Phytophthora effector target screening in yeast. This method may also be applied to other host plants and pathogen species.
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